The Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Twirling Filament Assay
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The polarized total internal reflection fluorescence microscopy (polTIRFM) twirling filament assay.
Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single molecules. polTIRFM determines the three-dimensional angular orientation and the extent of wobble of a fluorescent probe bound to the macromolecule of interest. This protocol describes the twirling filament assay, so named because actin sometimes...
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ژورنال
عنوان ژورنال: Cold Spring Harbor Protocols
سال: 2012
ISSN: 1559-6095
DOI: 10.1101/pdb.prot069401